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化学专业英语文献翻译

来源:未知 作者:佚名 日期:2019-09-21 浏览:58

化学英语在线翻译_在线有道翻译英语_英语在线语音翻译

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英语文献翻译

学院:化学化工学院

班级:应化1106

在线有道翻译英语_化学英语在线翻译_英语在线语音翻译

姓名:辛友伶

吴海军

学号:20110222234

20110222227

化学英语在线翻译_在线有道翻译英语_英语在线语音翻译

Quantifying the Cluster of Differentiation 4 Receptor Density on Human T Lymphocytes Using Multiple Reaction Monitoring Mass Spectrometry

多反应监测质量光谱法应用于人类T淋巴细胞量化分化抗原簇4受体密度

ABSTRACT: Cluster of differentiation 4 (CD4) is an important glycoprotein containing four extracellular domains, a transmembrane portion and a short intracellular tail。 It locates on the surface of various types of immune cells and performs a critical role in multiple cellular functions such as signal amplification and activation of T cells。 It is well-known as a clinical cell surface protein marker for study of HIV progression and for defining the T helper cell population in immunological applications。

Moreover, CD4 protein has been used as a biological calibrator for quantification of other surface and intracellular proteins。 However, flow cytometry, the conventional method of quantification of the CD4 density on the T cell surface depends on antibodies and has suffered from variables such as antibody clones, the fluorophore and conjugation chemistries, the fixation conditions, and the flow cytometric quantification methodsused。 In this study, we report the development of a highly reproducible nano liquid chromatographymultiple reaction monitoring mass spectrometry-based quantitative method to quantify the CD4 receptor density in units of copy number per cell on human CD4+ T cells。 The method utilizes stable isotope-labeled full-length standard CD4 as an internal standard to measure

化学英语在线翻译_在线有道翻译英语_英语在线语音翻译

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Quantifying the Cluster of Differentiation 4 Receptor Density on Human T Lymphocytes Using Multiple Reaction Monitoring Mass Spectrometry

多反应监测质量光谱法应用于人类T淋巴细胞量化分化抗原簇4受体密度

ABSTRACT: Cluster of differentiation 4 (CD4) is an important glycoprotein containing four extracellular domains, a transmembrane portion and a short intracellular tail。 It locates on the surface of various types of immune cells and performs a critical role in multiple cellular functions such as signal amplification and activation of T cells。 It is well-known as a clinical cell surface protein marker for study of HIV progression and for defining the T helper cell population in immunological applications。

化学英语在线翻译_在线有道翻译英语_英语在线语音翻译

Moreover, CD4 protein has been used as a biological calibrator for quantification of other surface and intracellular proteins。 However, flow cytometry, the conventional method of quantification of the CD4 density on the T cell surface depends on antibodies and has suffered from variables such as antibody clones, the fluorophore and conjugation chemistries, the fixation conditions, and the flow cytometric quantification methods used。 In this study, we report the development of a highly reproducible nano liquid chromatographymultiple reaction monitoring mass spectrometry-based quantitative method to quantify the CD4 receptor density in units of copy number per cell on human CD4+ T cells。 The method utilizes stable isotope-labeled full-length standard CD4 as an internal standard to measure

endogenous CD4 directly, without the use of antibodies. The development of the mass spectrometry-based approach of CD4 protein quantification is important as a complementary strategy to validate the analysis from the cytometry-based conventional method. It also provides new support for quantitative understanding and advanced characterization of CD4 on CD4+ T cells.

摘要:集群分化4(CD4)是一种重要的糖蛋白,它包含四个胞外区域,横跨膜的部分和短的细胞内尾巴。它位于各种类型免疫细胞的表面,在多种细胞功能中扮演重要角色,像细胞信号放大和激活的T细胞。众所周知的是作为研究艾滋病病程的临床细胞表面蛋白标记和在免疫学应用程序中定义辅助T细胞数量。除此之外化学英语在线翻译,CD4细胞蛋白质也已被用作其他表面和胞内蛋白量化的生物校准器。但是,流式细胞化学英语在线翻译,传统量化CD4 T细胞表面密度的方法取决于抗体和并且会受到像抗体克隆、荧光团和结合化学、固定条件以及以前流式细胞定量方法等变量带来的改变。在这项研究中,我们报道一种人类CD4 + T细胞中量化CD4受体密度在每个细胞的拷贝数的高度可再生的纳米液相色谱 - 多反应监测质谱为基础的定量方法的发展。该方法利用稳定同位素标记的全长标准CD4作为内部标准来直接衡量内源性CD4,而不需要使用抗体。 以CD4质谱为基础的蛋白定量方法的发展,作为一个重要的补充战略来验证分析以流式细胞仪为基础的传统方法。它还提供

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